Journal: Nutrients

Article Title: Anti-Remodeling Effects of Xanthohumol-Fortified Beer in Pulmonary Arterial Hypertension Mediated by ERK and AKT Inhibition

doi: 10.3390/nu11030583

Figure Lengend Snippet: Xanthohumol-fortified beer (FB) improved monocrotaline (MCT)-induced RV remodeling: ( a ) cardiomyocyte cross sectional area, ( b ) fibrosis (demonstrated by the % of collagen deposition), ( d ) VEGFR-2, and ( e ) p-ERK1/2/ERK1/2 ratio. Representative images from CSA and fibrosis from different experimental groups ( c ). Data are mean ± SEM from samples of eight animals per group. * p < 0.05 vs. respective control group, # p < 0.05 vs. Control + SHAM, † p < 0.05 vs. MCT + SHAM. RV: right ventricle, CSA: cross sectional area.

Article Snippet: Membranes were incubated overnight at 4 °C, with primary antibody solution diluted 1:1000 in 5% (w/v) nonfat dry milk or BSA in TBS-T (rabbit anti-p44/42 MAPK (ERK1/2), cell signaling #9102, rabbit anti-phospho-p44/42 MAPK (ERK1/2), cell signaling #9101; rabbit anti-AKT, cell signaling #9272; mouse anti-phospho-AKT (Ser473), cell signaling #4051; rabbit VEGF receptor (VEGFR)-2, cell signaling #9698; rabbit Caspase-3, cell signaling #9665, rabbit BCL-XL, cell signaling #2762, and rabbit BAX, cell signaling #2772).

Techniques: Control

Journal: The Journal of Experimental Medicine

Article Title: Induction of Apoptosis of Metastatic Mammary Carcinoma Cells In Vivo by Disruption of Tumor Cell Surface CD44 Function

doi:

Figure Lengend Snippet: ( A ) [ 3 H]-HA uptake and degradation. Soluble CD44 expression by the transfected TA3 cells results in a reduction of up to three-fold of 3 H-HA uptake and degradation. ( B ) Invasion of G8 myoblast monolayers by TA3neo and TA3sCD44 cells. TA3neo ( a and c ) and TA3sCD44v6-10 ( b and d ) cells (5 × 10 3 cells/well) were seeded onto DMSO-fixed G8 monolayers which were untreated ( a and b ) or treated with streptomyces hyaluronidase (10 U/ml, ICN, Costa Mesa, CA; c and d ) at 37°C for 3 h. 6 d after seeding of TA3 transfectants, TA3 neo cells are observed to penetrate and invade the monolayer, treated or not with hyaluronidase, and form tumor cell islets ( arrows , TA3 neo cells). By contrast, TA3sCD44 cells were unable to penetrate the untreated G8 monolayer ( b , arrowheads ), whereas treatment of the fixed G8 cells with hyaluronidase restored the ability of TA3sCD44 cell to penetrate the monolayer ( d , open arrows ).

Article Snippet: G8 mouse fetal myoblasts were obtained from American Type Culture Collection (ATCC, Rockville, MD), and cultured in DMEM with 10% fetal bovine serum (FBS, both from Irvine Scientific, Santa Ana, CA) and 10% horse serum ( GIBCO BRL , Gaithersburg, MD).

Techniques: Expressing, Transfection